MAPSI Key Features：
l Single-cell precision optogenetic stimulation;
l Calcium imaging and light stimulation (excitation/inhibition) synchronized;
l Calcium indicator + optogenetic actuator;
l Minimizes biological interference, maximizes SNR;
l Simultaneous tracking and behavior analysis;
l Ultra-wide field of view, up to 1mm wide;
l Resolve 1000+ neurons at single cell resolution;
l Track the same cells for months;
l Electronic focusing keeps the field of view stable;
l Image cortex, deep brain nuclei, brain stem, midbrain areas;
l Comes with software for rigid/non-rigid motion correction, CNMFe cell segmentation.
Range of view
Cells #1-5 selected for stimulation within field of view
Stimulate any combination of cells and observe responses of nearby neurons upstream and downstream.
A one-photon endoscope for simultaneous patterned optogenetic stimulation and calcium imaging in freely
behaving mice, Jingyong Zhang et al., (2022), Nature Biomedical Engineering. 7(4):1-12
Technical Specifications：
| Description | Specifications |
| Mode | Single-cell targeted optogenetics + single-photon microscope |
| Integrated LED light source | Yes |
| Objective NA | 0.6 |
| Field of view | 980 μm x 980 μm |
| Resolution | 800×600 pixels |
| Working distance | 50-500 μm |
| Auto-focus range | 0-300 μm |
| Auto-focus precision | < 0.5um axially |
| Frame rate | 5 - 60 FPS adjustable |
| Microscope dimensions | 8.8 mm x 19 mm x 23 mm |
| Weight | 4.5 g |
| Cable length | Customizable, 2.5m-3m |
| Imaging specifications |
| Fluorescence excitation | 550 ± 20 nm |
| Fluorescence power | 0 - 3 mW/mm2 |
| Fluorescence precision | 0.05 mW/mm2 |
| Fluorescence emission | 630 ± 37 nm |
| IR filtering | Filters out other wavelengths incl. IR |
| Optogenetics |
| Opto excitation | 470±20nm |
| Opto intensity | 0-35mW/mm² |
| Opto precision | 0.35mW/mm² |
| Opto pulses | 脉冲序列持续时间 :5ms 至 2h 脉冲宽度 :5ms 至 500ms |
| MAPSI |
| Opto resolution | 600*480 |
| Opto min. spot size | 10um diameter |
| Opto max. stimulation area | 600um*600um |
| Targeting precision | <10um |
| The number of neurons be selected | 80 |